Biomedical Research

Objective: The objective of this in-vitro study was to evaluate and compare the antimicrobial activity of three endodontic herbal irrigating agents, Propolis, Azadirachtaindica (neem) and Morinda citrifiolia (MCJ) against Actinomyces adicidentis.
Methods: Disk diffusion method was used. After the preparation of herbal medicine, few colonies were transferred in Trypticase Soy Broth (Hope Bio China) medium contained in a test tube and kept for overnight growth. Culture was centrifuged at 3000 rpm (Cence China) for 10 minutes and 0.5 McFarland standard prepared until optical density of 600 nm was achieved. The samples were collected and 1:10 serial dilution was prepared with it. 100 µL of the suspension was added to Columbia blood agar (Oxoid) plates by using streak method. Primary culture plates were incubated at 37? in 5% CO2 incubator for 24 hours. After 24 hour zone of inhibition was achieved, sample that were analyzed by counting the colony forming units. The ANOVA test was used for statistical analysis of the data. The level of significance was set at 0.05.
Result: A quantitative and qualitative analysis showed complete inhibition. Neem showed the highest zone of inhibition against Actinomyces radicidentis followed by sodium hypochlorite, Propolis and MCJ. Neem, propolis, MCJ and NaOCl showed a significant reduction (p <0.05) in the mean CFU counts for bacteria reduction.
Conclusion: An in vitro observation of herbs seems promising and is highly satisfactory. It seems herbs have better efficacy, bio compatibility, economics and easily available. NaOCL have several undesirable characteristic.

Author(s): Syed Yousif Ali shah, Hussain Iftikhar, Lei Chang, Yumei Niu, Shuang Pan
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