Biomedical Research

Objective: To investigate the expression of Thrombomodulin (TM) in lung tissue cells of model rats by using the ALI/ARDS rats induced by severe sepsis as a research model.

Methods: The ALI/ARDS (Acute Lung Injury/Acute Respiratory Distress Syndrome) model rats were prepared by the cecal ligation and puncture method in combination with 0.9% sodium chloride saline solution lavage, and 22 rats were randomly divided into the sham operation group and ALI/ARDS group. After successful modeling, rats were sacrificed in 1 h, 2 h and 3 h, and quantitative lung tissues were used to prepare the tissue homogenate, and then the expression of TM was measured by Enzyme- Linked Immunosorbent Assay (ELISA).

Results: The expressions of TM in the sham operation groups were (541 ± 15 ng/ml), (733 ± 27 ng/ml), (671 ± 34 ng/ml) at 1, 2, and 3 h respectively, in the ALI/ARDS group, the expressions of TM in 1 h, 2 h and 3 h after lavage were (16790 ± 155 ng/ml), (10034 ± 281 ng/ml) and (11698 ± 195 ng/ml), respectively. By comparison, the expression in the ALI/ARDS group was significantly higher than that of the control group, showing significant difference between groups (P<0.05). There was no significant difference in all time points in the groups (P>0.05), but the animals in the two groups showed a significant increase in stress at 1 h, and continued decrease at 2 h, and then increase at 3 h.

Conclusions: The expression of TM in the lung tissue cells of ALI/ARDS rats induced by severe sepsis was high, indicating that it has obvious effect of anticoagulation and wide application values in the clinical treatment, thus, it is worthy of promotion.

Author(s): Lei Lirong, Wu Yan, Zhan Jia, He Xianghu, Wang Yanlin
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