Biomedical Research

Objective: To analyse the quantitative differences of Eubacterium rectale, Bacteroides thetaiotaomicron, Lactobacillus and Bifidobacterium in non-alcoholic fatty liver patients with type 2 diabetes and nonalcoholic fatty liver patients with normal glucose metabolism to explore the relationship of intestinal flora and the occurrence and development of non-alcoholic fatty liver disease and diabetes.

Methods: 100 cases of Nonalcoholic Fatty Liver Disease (NAFLD) patients with type 2 diabetes, 50 cases of NAFLD patients with normal glucose metabolism and 60 cases of normal metabolism without NAFLD (normal control group) were selected as research objects. The total DNA of faeces was extracted and the expression level of DNA of target bacteria was detected by relative quantification of Real Time PCR.

Results: The quantity of Eubacterium rectale and Lactobacillus in NAFLD patients with type 2 diabetes group was significantly higher than that of NAFLD patients with normal glucose metabolism group, the difference of which was statistically significant (P=0.00, P=0.006), and Bacteroides thetaiotaomicron decreased significantly, the difference of which was statistically significant P=0.002), and the quantity of Bifidobacterium presented no significant difference (P>0.05). The quantity of Eubacterium rectale and Lactobacillus in NAFLD patients with type 2 diabetes group were significantly higher than that of normal control group, the difference of which was statistically significant (P=0.00), and Bacteroides thetaiotaomicron as well as Bifidobacterium decreased significantly, the difference of which was statistically significant (P=0.00). The quantity of Bifidobacterium in NAFLD patients with normal glucose metabolism group decreased significantly normal compared to normal control group, the difference of which was statistically significant (P=0.00), and Eubacterium rectale, Bacteroides thetaiotaomicron and Lactobacillus had no significant difference (P>0.05).

Conclusion: Intestinal flora in patients with non-alcoholic fatty liver disease of different glucose metabolism changes much.

Author(s): Jiang Yan, Xue Ping-yan, Xu Yu-shan, Yang Hui-ying, Li Hong, Zhang Wen-hua, Song Ya-xian, Liu Hua, Wang Xiao-e
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