Biomedical Research

The antibacterial resistant pattern of aerobic bacteria, isolate from burn patients admitted in plastic surgery & general surgery wards of Chhatrapati Shahuji Maharaj Medical Uni-versity, Lucknow (a tertiary care hospital) were studied. 100 patients were enrolled from plastic surgery & general surgery wards and 200 samples were collected which comprised of 100 burn wound swabs & 100 biopsies of same patients. All samples were cultured on Nutrient agar, Mac conkey agar and Blood agar at 37ºc for 24 hrs. The isolates were identified by culture, staining and biochemical tests including oxidase, lactose and maltose fermentation, catalase and their antibiotic sensitivity determined using Kirby Bauer disc diffusion technique. The most common isolate was Pseudomonas aeruginosa-55.0%, followed by Staphylococcus aureus-19.29%, Klebsiella spp.-11.43%, Acinetobacter spps-7.14%. Proteus spp 4.29%, Es-cherichia coli-2.85%. Resistance of S.aureus was 40% observed with Oxacillin & 84% to Erythromycin whereas all strains were susceptible to Vancomycin. We analyzed that pesu-domonas which was the commonest isolate was most resistant to Ceftazidime (70%) followed by Cefotaxime. Ciprofloxacin (55.5%) & Amikacin (54.0%) were found to be most effective antimicrobial agent (7, 11). Other Gram-negative organisms were highly resistant to Cefo-taxime (66.0%) followed by Gentamycin (60.0%).). Imipenem was found to be less resistant (26%) against Pseudomonas.

Author(s): Anuradha Rajput, K.P.Singh, Vijay Kumar, Rishi Sexena, R.K.Singh
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