Biomedical Research

Background: Titanium (Ti) and Ti alloys are known to have good mechanical properties with high corrosion resistance and excellent biocompatibility. Doping of silver ions onto a titanium dioxide based surface resulted in the movement of the absorption to a longer wavelength due to a change in the electronic and optical properties of TiO₂ and thereby increasing the antibacterial effect.

Aim: This work was done to investigate the effectivity of nanoparticles of TiO₂ doped with 0.3% wt of silver prepared by sol-gel technique, as an anti-bacterial agent against Pseudomonas aeruginosa from clinical samples.

Materials and Methods: TiO₂ with 0.3% silver as additive was systematically assessed on 25 Pseudomonas aeruginosa isolates from various clinical samples and incubated in dark, in visible light and under UV radiation for the antibacterial effect. After each hour of incubation, the isolates were inoculated onto Mueller Hinton agar.

Results: The viable colonies diminished within 2 h of incubation under UV irradiation, while under visible light, the number of viable colonies was highly reduced after 3 h. Under dark, the reduction was considerably slower, taking 6 h, while in all the light conditions the control, which had no exposure to nanoparticles, showed no reduction in the number of colonies.

Conclusion: TiO₂ with Ag as an additive is highly effective as an antibacterial agent on many human pathogens including Pseudomonas aeruginosa, which is notorious in causing hospital acquired infections. TiO₂ with 0.3 wt (%) Ag is most effective under UV irradiation and least in dark.

Author(s): Hima Bindu M, Indu Kapur, Vijayaraghavan R
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