Effects of enzymatic digestion, cell culture and preservation conditions on surface CD62L expression of primary murine CD3+CD4+ T cells

Qi Liu; Rong Zhao; Chun Huang; Kai-Liang Zhou; Xiao-Lian Zhang; Qin Pan

doi:10.4066/biomedicalresearch.29-17-3070

Abstract

Effects of enzymatic digestion, cell culture and preservation conditions on surface CD62L expression of primary murine CD3+CD4+ T cells

Surface markers are used to identify distinct cell populations. CD62L, a cell adhesion molecule on leukocytes, is used to identify and segregate central memory T (T_CM) and effector memory T (T_EM) cells. In the present study, we evaluated the effects of experimental conditions on surface CD62L expression of primary murine CD3+CD4+ T cells. We found that CD62L expression levels were more resistant to collagenase D treatment. Prolonged culture (at 37°C) and preservation (at 4°C) periods resulted in modulation of surface CD62L expression in vitro. These results provide valuable information for the development and optimization of a reliable method for detecting of CD62L expression in vitro.

Author(s): Qi Liu, Rong Zhao, Chun Huang, Kai-Liang Zhou, Xiao-Lian Zhang, Qin Pan
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