Biomedical Research

Background: Through establishing a retina ischemia-reperfusion injury (RIRI) model, we observed the expression of inflammatory cytokines in retina to explore its possible role in the process of RIRI.

Methods: 75 healthy SD rats without eye disease were randomly divided into normal control group (NCG) and model group (MG).

Results: Protein expression of tumor necrosis factor-α (TNF-α), interleukin-23 (IL-23) and interleukin-17 (IL-17) enhanced obviously in RIRI retina. The TNF-α and IL-23 increased the most at 24 h after modeling, while IL-17 reached the peak at 72 h after modeling. RT-qPCR results showed that 12, 24, 72 and 144 hours after modeling, mRNA expression of TNF-α, IL-23 and IL-17 increased obviously in MG. The mRNA expression of TNF-α and IL-23 increased the most at 24 h after modeling, while IL-17 reached the peak at 72 h after modeling. The difference was significant (P<0.01).

Conclusions: Therefore, expression of TNF-α, IL-23 and IL-17 in retina enhanced obviously in RIRI SD rats. TNF-α, IL-23 and IL-17 can be viewed as pathogenic factors, involving in the pathological damage of RIRI through increasing retinal inflammation.

Author(s): Qinglei Song, Dan Liu, Haijiang Zhang, Liqun Yi, Ming Huo
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