Biomedical Research

Objective: This study aims to preliminarily investigate mechanisms of miR-100 in inhibiting invasion and migration of nasopharyngeal carcinoma cells by regulating ZBTB7A gene.

Methods: The nasopharyngeal carcinoma cell line CNE-2 was cultured and inoculated in a 12-well plate. Cells were divided into the overexpression group, interference group, and mock group, with four complex holes in each group. The medium was cultured for 24 h after every experiment. Invasive cells passing through Matrigel were detected using Transwell chamber, whereas cell migration rate of each group was recognized by conducting a 48 h scratch test. Meanwhile, expressions of miR-100 and ZBTB7A mRNA in each group were identified by using fluorescent quantitative polymerase chain reaction, whereas expression of ZBTB7A protein in each group was distinguished by administering Western blot.

Results: The number of invasive cells passing through Matrigel in the overexpression group was significantly lower than that of the interference and mock groups (P<0.05). Cell migration rate in the overexpression group was significantly lower (P<0.05) and showed no significant difference (P>0.05) with the remaining groups. Relative expression of miR-100 mRNA was the highest in the overexpression group (P<0.05), whereas that in the interference group was significantly lower than that of the mock group (P<0.05). Both expressions of ZBTB7A mRNA and protein in the overexpression group were significantly lower than those of the interference and mock groups (P<0.05). Expressions of ZBTB7A mRNA and protein in the overexpression group were significantly higher than those of the interference and mock groups (P<0.05).

Conclusion: We speculated that miR-100 inhibits invasion and migration of nasopharyngeal carcinoma cells through regulating ZBTB7A gene. However, the specific mechanism needs further study.

Author(s): Yi-Gang Zhao, Xian-Zhi Wang
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