Biomedical Research

We investigated the effect of fat meal load (FML) on inflammatory factors during the process of atherosclerosis (ATH). Ten male New Zealand white rabbits (fed with high-fat meal for 12 weeks, and provided FML at the beginning (W1), as well as in the 4^th, 8^th, and 12^th weeks, (W4, W8, and W12, respectively) were used in this study. Blood samples were collected after fasting and at 2, 4, 6, and 8 h after FML (T0, T1, T2, T3, and T4, respectively) to detect plasma endothelin-1 and interleukin (IL)-6, IL-8, and IL-10; ATH conditions in the abdominal aorta were observed ultrasonically. At W12, the animals were sacrificed and ATH conditions were detected by hematoxylin and eosin staining. After continuous high-fat feeding, fasting plasma endothelin-1 was increased in W4, W8, and W12, IL-8 was increased in W4 and IL-10 was increased in W4 and W8. After FML, endothelin-1 was significantly increased at W0-T2, and W12-T1, IL-8 was increased at W0-T1-3, W4-T1-2, and W8-T1-3, and IL-10 was increased at W0-T1-4, W4-T2-4, and W12-T1-2. During early ATH, inflammation was severe and clearly observed at 4-6 h after FML.

Author(s): Chunrong Jin, Xue Zhou, Shuling Yang, Xiaoli Zheng, Sijin Li
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