Biomedical Research

The aim of this study was to investigate the proliferation, adhesion, and migration function of chloride ion channels in HLEC B-3, a human lens epithelial cell line, and provide experimental basis for studying the mechanism of human after-cataract as well as the effective methods for controlling and treating it. MTT and Scratch assay methods were applied to detect the proliferation, adhesion, and migration of HLEC B-3 after applying 5, 10, 20, 50, 100, and 200 μmol/L of chloride ion channel blockers (NPPB). In the cell proliferation experiment, no apparent difference was found in the first 12 and 24 h after treatment with 10, 20, 50, 100 and 200 μmol/L NPPB or 48 h after treatment with 20, 50, 100 and 200 μmol/L NPPB. The cell proliferations were obviously inhibited compared with the control group(without NPPB treatment), and the difference was significant (P < 0.05 or P < 0.01). Cell adhesion experimental results showed that when the cells were treated with 10, 20, 50, 100, and 200 μmol/L NPPB for 12 or 24 h, cell adhesions were obviously inhibited. The difference was very significant (P < 0.05 or P < 0.01) compared with the control group. The activity of chloride ion channels can inhibit the proliferation, adhesion, and migration of LECs; therefore, inhibiting its activity can control the occurrence of human after-cataracts.

Author(s): Gui-Rong Li, Qiong-Shu Li, Xin-Yue Yang, Miao-Miao Liu, Yue Cheng, Rui Fei, Ya-Ping Li
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