Biomedical Research

This study was conducted to investigate the free radicals scavenging activity and cytoprotective effects of Lactobacillus plantarum YS-1 Fermented Soy Milk (FSM-LPYS1) in Caco-2 human colon adenocarcinoma cells damaged by hydrogen peroxide (H₂O₂)-induced oxidation. FSM-LPYS1 showed a remarkable in vitro activity against 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH) and hydroxyl radical (•OH). FSM-LPYS1 significantly decreased the levels of intracellular Reactive Oxygen Species (ROS), lipid peroxidation, and enhanced the activities and expression levels of Catalase (CAT), Superoxide Dismutase (SOD) and glutathione peroxidase (GSH-Px) in H₂O₂-treated Caco-2 cells (p<0.05). FSM-LPYS1 also reduced the protein levels of IL-8 and NF-κB in Caco-2 cells. These results clearly indicated that FSMLPYS1 had impressively scavenged free radicals and were able to reduce the H₂O₂-induced oxidative damage in Caco-2 cells by reducing the intracellular ROS generation, restraining lipid peroxidation, and by raising the endogenous antioxidant enzyme activity. In addition, the FSM-LPYS1 could also reduce the H₂O₂-induced inflammation reaction. FSM-LPYS1 is a functional food which has antioxidant properties and it can be used as high quality soybean processing food for exploitation of health benefits.

Author(s): Guijie Li, Xingyao Long, Yanni Pan, Xin Zhao, Jia-Le Song
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