Biomedical Research

Objective: To investigate the effects of Hypoxia Inducible Factor-1 alpha (HIF-1α) and Vascular Endothelial Growth Factor (VEGF) on wound healing of diabetic mice.

Methods: Streptozotocin (STZ) was used to induce establishing wound healing model group of type 1 diabetic mouse in the experiment. The protein expression of HIF-1α and VEGF at different time point were measured in the wound healing model group of type-I diabetic mouse and normal control group by use of Western blotting and then relative mRNA expression quantity of HIF-1α and VEGF were determined with relative quantitative method.

Results: Before the operation, the difference between the diabetic model group and the normal control group in HIF-1α and VEGF expression as well as the relative expression quantity of mRNA was not statistically significant (P>0.05). At the fifth day of finishing operation, compared with the preoperative level the protein expression levels of HIF-1α and VEGF increased in the normal control group (P<0.05) and decreased in the diabetic model group (P<0.05) and the relative expression quantity of HIF-1α and VEGF mRNA in the normal control group was obviously higher that in the diabetic model group (P<0.05).

Conclusion: HIF-1α and VEGF play important roles in diabetic wound healing and the decrease of their expression level may lead to lack of angiogenesis, which will delay the healing of diabetic wound.

Author(s): Chujia Lin, Guoshu Yin, Miaoqiong Ou, Shaoyu Zheng
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