Biomedical Research

Purpose: We evaluated accuracy and effect of ultrasound (US)-guided transcranial drilling and catheter drainage versus computed tomography (CT)-controlled interventions in the collagenase-induced intracerebral haemorrhage in rats.

Method: 120 male Sprague Dawley (SD) rats were randomly divided into four groups: group A, group N, group B and group C. Group A, group B and group N underwent collagenase-induced Intracerebral Haemorrhage (ICH) surgery procedure as animal model groups. Group C received a 10 μl saline injection with the same surgical procedure as the control group. US-guided transcranial drilling and catheter drainage were performed on group A. CT-controlled transcranial drilling and catheter drainage were performed on group B. Then group A, group B and group N were evaluated by ultrasound and CT. All groups were observed and assessed the behavior tests and mortality for 4 weeks. The remains of all groups were put to death after assessment. Apoptosis of neuron cells were identified by hematoxylin and eosin stain and immunohistochemistry.

Results: The mortality of each group showed no significant difference (p>0.05). Behavioral testing showed that there was no significant difference between group A and B (p>0.05). The expression of NFkB and caspase-3 revealed that there was a few apoptosis of neuron cells both in group A and B.

Conclusions: US-guided transcranial drilling and catheter drainage are as accurate as CT-controlled transcranial drilling and catheter drainage. It could result in a significant reduction of procedure expenditure under the avoidance of radiation and show the same therapeutic effect.

Author(s): Li Chen, Xuan Zhou, Cong Feng, Xiang Cui, Lili Wang, Tanshi Li
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