Biomedical Research

ANTI-HYPERGLYCEMIC ACTION OF GYNURA PROCUMBENS IS PARTLY MEDIATED BY INHIBITION OF CARBOHYDRATE DIGESTION AND ABSORPTION IN THE GUT

Joint Event on 3^rd INTERNATIONAL OBESITY SUMMIT AND EXPO & 2^nd International Conference on DIABETES, NUTRITION, METABOLISM & MEDICARE & World Conference on LASER, OPTICS AND PHOTONICS
November 05 -06 , 2018 | Philadelphia , USA

Kazi Ishtiaq Ahmad, Mahbub Hasan, Limu Parvin, Afra Haque, Kamol Krishna Nandy and JMA Hannan

Independent University, Bangladesh

Scientific Tracks Abstracts : Biomed Res

DOI: 10.4066/biomedicalresearch-C7-019

Abstract:

Gynura procumbens leaf has been widely used as a traditional therapy for diabetes all over the world. Present study aims to investigate effects of G. procumbens in sucrose digestion and absorption in the gut to explore the anti-hyperglycemic activity of this plant. The dried-powder-leaves of G. procumbens were extracted with methanol. Sucrose malabsorption in GI Tract was evaluated in 20-hrs-fasted Long Evan rats by determining the amount of sucrose remaining in six different parts of gastrointestinal tract after sucrose load (2.5 g/kg b.wt), with or without 500 mg/kg dose of G procumbens extract. For evaluation of disaccharides activity, the extract was fed to 20-h-fasted rats. After 60 min, the rats were sacrificed, and the small intestines were isolated and homogenized. The homogenate (20μl) was incubated for 60 min at 37 °C with 40 mmol sucrose. Disaccharides activity was calculated by glucose converted from sucrose as mol-mg glucose/protein/h. When the extract of G procumbens was administered simultaneously with the sucrose load, the residual sucrose content in the gastrointestinal tract was increased significantly (p<0.01), especially in the upper intestine at 30 min, in the whole intestine as well as cecum at 1 and 2 h. At 4 h, sucrose was not detected in the gut in both groups. When extract was supplemented with the glucose solution, the percentage absorption of glucose was decreased by 13-19% during whole perfusion period (p<0.05). It inhibited disaccharides (sucrose) activity significantly (p<0.05) in rats. The anti-hyperglycemic activities of G procumbens in rats are partly mediated via delaying intestinal carbohydrate digestion and absorption.

Biography:

Kazi Ishtiaq Ahmad has recently completed masters of pharmacy, major in clinical pharmacy and molecular pharmacology from East West University, Dhaka, Bangladesh and completed bachelor of pharmacy from BGC Trust University, Bangladesh. He has completed his M Pharm research under the active supervision of JMA Hannan in the field of diabetes.

E-mail: kazilingkon@gmail.com

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