Biomedical Research

INVESTIGATING THE CELLULAR DYNAMICS OF ORGANS DEVELOPMENT AND CANCER USING 3D IMAGING

Joint Event on International Conference on CARDIOLOGY AND CARDIOVASCULAR MEDICINE & International Conference on STEM CELLS AND REGENERATIVE MEDICINE
June 18-19, 2018 | Osaka, Japan

Anne Rios

Princess Maxima Center, Netherlands

Posters & Accepted Abstracts : Biomed Res

DOI: 10.4066/biomedicalresearch-C2-006

Abstract:

In collaboration with her colleague Nai Yang Fu, I implemented a novel 3D-imaging approach (with 3D glasses) to perform innovative multicoloured lineage tracing studies to follow the development and fate of mammary stem cells (MaSC) and descendant progenitor cells in vivo in entire mammary gland. As stem cells divide they produce clones of cells; using this imaging technique the fate of these individual clones could be tracked throughout various stages of mammary gland development, including puberty, pregnancy and normal adult homeostasis. This work provided the first in vivo evidence for the existence of bipotent MaSCs, which give rise to the two cell lineages that constitute the mammary ducts, the luminal and the myoepithelial cells, as well as the presence of distinct long-lived unipotent progenitor cells. The cellular dynamics observed at different developmental stages support a model in which both stem and progenitor cells drive morphogenesis during puberty, whereas bipotent MaSCs coordinate ductal homeostasis and remodelling of the adult mouse gland (Nature 2014, Nature Comm. 2016, NCB 2017). We have now specialized this 3D technology to detect early aberrant cellular behaviour in models of breast cancer and to visualise how cancerous cells, according to their cell-of-origin, exit normal ductal homeostasis and survive to self-organise into a solid tumour.

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