Biomedical Research

Maintaining physiological fidelity during in vitro stem cell culture and expansion; a role for oxygen control

Joint Event on 2^nd International Conference on Tissue Science and Molecular Biology, Stem Cells & Separation Techniques
June 06-07, 2019 | London, UK

Nicholas R Forsyth

Keele University, UK

Posters & Accepted Abstracts : Biomed Res

Abstract:

Hypoxia or physiological normoxia, plays a key role in determining stem cell behaviour in the in vivo niche. In spite of this little attention is payed to the role of reduced oxygen levels during in vitro culture generating a risk of forced paradigm and artefactual norms. Bone marrowderived human mesenchymal stem cells (hMSC), due to the sinusoidal blood vessel architecture found within their niche, are particularly vulnerable to oxygen tension fluctuations. We and others, have now described fundamental, artefactual, alterations in hMSC biology as a consequence of air oxygen exposure. These include reduced colony forming unitfibroblastic isolation; dysregulated epigenome, transcriptome and proteome; altered biochemical volatile footprints during culture; and counter intuitive alterations in reactive oxygen species management. This lecture will discuss the fundamental biology underpinning these biological differences, their potential impact on regenerative medicine and what we can do to transform biological understanding into therapeutic application.

Biography:

E-mail:

n.r.forsyth@keele.ac.uk

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