Biomedical Research

SOME IMPORTANT CONCEPTS FOR LEAF PROTEIN EXTRACTION AND TWO-DIMENSIONAL ELECTROPHORESIS TECHNIQUE

Joint Event on Global Congress on BIOTECHNOLOGY & Annual Congress on EMERGING MATERIALS AND NANOTECHNOLOGY
September 06-07 , 2018 | Bangkok , Thailand

Daryush Talei

Shahed University, Iran

Posters & Accepted Abstracts : Biomed Res

DOI: 10.4066/biomedicalresearch-C4-011

Abstract:

Proteomic analysis of plants relies on high yields of pure protein. In plants, protein extraction and purification to perform two-dimensional electrophoresis technique present a great challenge due to accumulation of a large amount of interfering substances including polysaccharides, polyphenols and secondary metabolites. Therefore, it is necessary to modify and use an effective protein extraction protocols for two-dimensional electrophoresis technique. To achieve high yields of pure protein and successful twodimensional electrophoresis some important factors during protein extraction process, protein precipitation methods, isoelectric focusing program such as; rehydration types (passive or active), isoelectric point (PI), IPG strips (size and pH), power conditions and resolution in IEF, equilibration buffer, SDS-PAGE process such as; choosing a gel size format, gels percentage and power conditions, detection of protein in gels, image achievement and analysis, and identification and characterization of 2-D protein spots should be considered.

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